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This disclosure relates to method of isolating rare cells, e.g. unique cancer cells. In certain embodiments, the disclosure contemplates methods of identifying unique cells that are in a sample that contains a group of cells that are replicating at unique locations and/or at different rates. In certain embodiments the uniquely isolated and/or identified cells are evaluated for genetic content and/or expression for diagnostic evaluations. In certain embodiments, the disclosure contemplates compositions comprising cells that are derived from methods disclosed herein.