RARE MUTATION DETECTION METHOD, DETECTION DEVICE, AND COMPUTER PROGRAM

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PROBLEM TO BE SOLVED : To provide methods of detecting a rare mutation in a template DNA in distinction from the mutation caused by the errors in a nucleic acid amplification and nucleic acid sequence analysis, and to provide an apparatus and computer program for performing the method. SOLUTION : A method comprises the steps of : analyzing the nucleic acid sequence of a library produced by a nucleic acid amplification reaction using a sample containing a template DNA of 1000 copies or less; calculating the mutant ratio in a base at a given position from the analyzed results; and comparing the calculated mutant rate with a given cutoff value to detect a rare mutation. SELECTED DRAWING : None COPYRIGHT : (C)2017, JPO&INPIT