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PROBLEM TO BE SOLVED : To provide novel tetrakis(carboxyalkyl)glycolurils and application thereof, especially a crosslinking agent for an epoxy resin and a manufacturing method thereof.SOLUTION : The present invention provides 1, 3, 4, 6-tetrakis(carboxyalkyl)glycolurils represented by the general formula (I), where n is 0 or 1, and Rand Rare each independently H or a lower alkyl group.