ANTIBODY-BASED ARRAYS FOR DETECTING MULTIPLE SIGNAL TRANSDUCERS IN RARE CIRCULATING CELLS

文件类型：PDF文档
文件大小：4430K
The present invention provides antibody-based arrays for detecting the activation state and/or total amount of a plurality of signal transduction molecules in rare circulating cells and methods of use thereof for facilitating cancer prognosis and diagnosis and the design of personalized, targeted therapies. In the methods of the present invention, a plurality of activation state-independent antibodies are labeled with a facilitating moiety such as glucose oxidase, and a plurality of activation state-dependent antibodies are labeled with a first member of a signal amplification pair such as a peroxidase, wherein the facilitating moiety generates an oxidizing agent such as hydrogen peroxide (H2O2), which channels to and reacts with the first member of the signal amplification pair. The methods allows for detecting the activation state and/or total amount of an analyte.