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A preparation method of rare earth ions doped alkaline earth metal silicate luminescent glass is provided. The steps involve : step 1, mixing the source compounds of cerium, terbium and alkaline earth metals and putting the mixture into solvent to get a mixed solution; step 2, impregnating the nanometer pores glass with the mixed solution obtained in step 1; step 3 : calcining the impregnated nanometer pores glass obtained in step 2 in a reducing atmosphere, cooling to room temperature, then obtaining the cerium and terbium co-doped alkaline earth metal silicate luminescent glass. Besides, the rare earth ions doped alkaline earth metal silicate luminescent glass prepared with aforesaid method is also provided. In the prepared luminescent glass, cerium ions can transmit absorbed energy to terbium ions under the excitation of UV light due to the co-doping of cerium ions. As a result, the said luminescent glass has higher luminous intensity than the glass only doped with terbium.