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The present invention provides a method for producing an oxo fatty acid and a rare fatty acid, such as a conjugated fatty acid, a hydroxylated fatty acid, or a partially saturated fatty acid, using four types of enzymes (fatty acid hydratase, hydroxylated fatty acid dehydrogenase, oxo fatty acid isomerase, and oxo fatty acid enone reductase) derived from, for instance, a lactobacillus such as Lactobacillus plantarum. The present invention further provides a method for the more efficient production of an oxo fatty acid and the like by the partial combined use of chemical oxidation.