MULTIPLEX REAL TIME PCR METHOD FOR DISCRIMINATION OF RICE CULTIVAR

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PURPOSE : A method for identifying Oryza sativa species using real time multiple PCR is provided to shorten PCR process and to lower probability of error occurrence. CONSTITUTION : A marker complex composition for identifying Oryza sativa species contains two selected from : probes of sequence numbers 1, 2, and 3; probes of sequence numbers 4, 5, and 6; probes of sequence number 7, 8, and 9; probes of sequence numbers 10, 11, and 12; probes of sequence numbers 13, 14, and 15; probes of sequence numbers 16, 17, and 18; probes of sequence numbers 19, 20, and 21; probes of sequence numbers 22, 23, and 24; probes of sequence numbers 25, 26, and 27; probes of sequence numbers 28, 29, and 30; probes of sequence numbers 31, 32, and 33; and probes of sequence number 34, 35, and 36.