Method For Producing Cloned Wolf

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PURPOSE : A method for producing a cloned wolf is provided to contribute to preserve a rare and endangered species wolf and develop veterinary medicine, anthropology and medicine study fields.CONSTITUTION : A method for preparing a nuclear transplant embryo of wolf comprises the steps of : (a) removing a nucleus from ovum of a canine animal such as dog, wolf, fox, jackal, coyote, dhole, and raccoon dog to prepare a denucleated ovum; (b) isolating a somatic cell from a tissue of the wolf to prepare a donor nucleus cell; (c) micro-injecting the donor nucleus cell into the denucleated ovum and electrofusing it; and (d) activating the fused ovum. A nuclear transplant embryo prepared by the method is deposited as a deposition no. KCTC 10903BP. A method for producing a cloned wolf comprises a step of transplanting the nuclear transplant embryo into an oviduct of a canine animal surrogate mother. Further, the electrofusing step is performed at a voltage of 3.6 to 4.1 kv/cm, for 10 to 30 μs, 1 to 3 times.? KIPO 2008