Homogeneous detection and/or determination method for a chemical or physico-chemical interaction

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The invention relates to a homogeneous method for detecting and/or determining by fluorescence a chemical or physicochemical interaction in a measuring medium, in which a rare-earth metal cryptate comprising a substituent is used, characterized in that the variation of at least one fluorescence characteristic of the rare-earth metal cryptate, induced by the change in the physicochemical properties of the substituent resulting from said interaction, is measured.