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BOTTLENECK SEQUENCING 发明申请

2023-08-03 1380 2382K 0

专利信息

申请日期 2025-07-09 申请号 WOUS17015229
公开(公告)号 WO2017132438A1 公开(公告)日 2017-08-03
公开国别 WO 申请人省市代码 全国
申请人 THE JOHNS HOPKINS UNIVERSITY
简介 Bottleneck Sequencing System (BotSeqS) is a next-generation sequencing method that simultaneously quantifies rare somatic point mutations across the mitochondrial and nuclear genomes. BotSeqS combines molecular barcoding with a simple dilution step immediately prior to library amplification. BotSeqS can be used to show age and tissue-dependent accumulations of rare mutations and demonstrate that somatic mutational burden in normal tissues can vary by several orders of magnitude, depending on biologic and environmental factors. BotSeqS has been used to show major differences between the mutational patterns of the mitochondrial and nuclear genomes in normal tissues. Lastly, BotSeqS has shown that the mutation spectra of normal tissues were different from each other, but similar to those of the cancers that arose in them.


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