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CELL-SPREADING METHOD AND CELL-SPREADING KIT FOR OBSERVING RARE CELLS 发明申请

2023-09-29 4770 2066K 0

专利信息

申请日期 2025-06-24 申请号 WOJP16081512
公开(公告)号 WO2017073533A1 公开(公告)日 2017-05-04
公开国别 WO 申请人省市代码 全国
申请人 KONICA MINOLTA INC
简介 The present invention addresses the problem of providing a means for increasing the efficiency of recovering rare cells in a cell suspension by suppressing adsorption of the cells to the experimental equipment without inhibiting adsorption of the cells to a cell-spreading substrate. A cell-spreading method according to the present invention includes : a step for adding protein to a cell suspension in such an amount that the final concentration is 0.0001-0.1 %, or, preferably, 0.0005-0.01 % (protein adding step); and a step for making cells in the cell suspension, which has been subjected to the protein adding step, spread on, stand still on, and adsorb to a cell-spreading substrate in which the contact angle with water is equal to or greater than 60º or, preferably, 70-90º respect to (cell spreading step).


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