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DETERMINING THE LIMIT OF DETECTION OF RARE TARGETS USING DIGITAL PCR 发明申请

2023-08-20 1220 1640K 0

专利信息

申请日期 2025-07-13 申请号 WOUS16035870
公开(公告)号 WO2016197028A1 公开(公告)日 2016-12-08
公开国别 WO 申请人省市代码 全国
申请人 LIFE TECHNOLOGIES CORPORATION
简介 A method for determining false positives calls in a biological data plot is provided. The method includes identifying a first data cluster as non-amplification data points within the biological data plot and identifying a second data cluster as wild-type positives within the biological data plot. The method further includes estimating a position in the biological data plot of a center of the first and second data clusters. The method further includes determining, for each data point within the first data cluster, a probability of belonging to the first data cluster and determining, for each data point within the second data cluster, a probability of belonging to the second data cluster. The method includes applying a probability threshold for each data point within the first and second data cluster to identify false positives.


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