| 简介 |
An objective of the present invention is to provide the cytolethal distending toxin (CDT) of C . hyointestinalis and polynucleotides encoding it, and novel methods for detection of C . hyointestinalis using the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) of Campylobacter bacteria, and detected the cdt genes of a Campylobacter -like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in C. jejuni , C. coli, and C . fetus, but not by multiplex PCR that can specifically detect the cdtA , cdtB , and cdtC genes of the three bacterial species. The bacterial strain was identified as C . hyointestinalis by 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene. |
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