| 简介 |
Disclosed are methods and systems for designing and using organism-specific and/or operational taxon unit (OTU)-specific probes for detecting, identifying and quantitating a plurality of biomolecules or rnicrorganisms in a sample based on the hybridization or binding of target molecules in the sample with the probes. The disclosure further provides methods of selecting an oligonucleotide probe specific for a node on a clustering tree, and methods of selecting organism-specific or OTU-specific oligonucleotide probes for use in accurately detecting a plurality of organisms in a sample with high confidence. Further, the disclosure provides methods and systems to detect the presence of a rare OTU in a sample. |
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