| 简介 |
It is intended to provide an artificial actinomyces H+-PPase gene derived from wild type actinomyces H+-PPase gene by partial modification of the base sequence thereof to thereby construct an expression system of Escherichia coli having the actinomyces H+-PPase gene integrating therein and provide a method of efficiently producing actinomyces H+-PPase. An artificial actinomyces H+-PPase gene derived from the sequence of wild type actinomyces H+-PPase gene by substituting a rare codon for of Escherichia coliby another codon in such a manner as not changing the amino acid sequence encoded thereby. The thus modified artificial actinomyces H+-PPase gene can stably exist on a plasmid inof Escherichia coli and its transformant can similarly grow to Escherichia coli. The above-described artificial actinomyces H+-PPase gene enables mass production of actinomyces H+-PPase that has been impossible hitherto. |
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