| 简介 |
PROBLEM TO BE SOLVED : To establish a reaction system for producing a plurality of kinds of rare sugars by reacting a plurality of kinds of rare aldoses and obtaining the most efficient isomerase for producing a plurality of kinds of rare ketoses in the strategy of the rare sugars of Izumoring (shown in the figure).
SOLUTION : A DNA encodes the following protein (a) or (b). (a) A protein composed of an amino acid sequence represented by sequence number 2 or (b) a protein composed of an amino acid sequence in which one or several amino acids are deleted, substituted or added in the amino acid sequence represented by the sequence number 2 and having an L-rhamnose isomerase activity. The DNA is the L-rhamnose isomerase derived from Pseudomonas stutzerii. The protein is composed of the amino acid sequence represented by the sequence number 2. A method for producing a recombinant protein comprises culturing a host cell containing an expression system expressing the protein in a culture medium and collecting the recombinant protein having the L-rhamnose isomerase activity from the resultant cultured product. A method is to utilize the figure for producing the rare sugars by understanding the positions of the objective rare sugars in monosaccharides in the whole image and design the optimum production route thereof for reacting the protein. |
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