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An object of the present invention is to provide a means that enables improvement of efficiency in recovery of rare cells in a cell suspension by suppressing cell adsorption to laboratory equipment but not suppressing cell adsorption to a cell spreading substrate. A cell spreading method according to the invention includes a step of adding to a cell suspension a protein in such an amount as to give a final concentration of from 0.0001% to 0.1%, and preferably from 0.0005% to 0.01% (a protein addition step) and a step of spreading cells contained in the cell suspension that has been subjected to the protein addition step on a cell spreading substrate having a contact angle with water of 60 degrees or more, and preferably from 70 to 90 degrees, and allowing to stand to cause the cells to adsorb onto the substrate (a cell spreading step).