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The present invention relates to a reusable system for detecting biomolecules, comprising : a detection device where collecting anti-body is fixed on a substrate; ferromagnetic nanoparticles having detection antibody; and rare-earth metal oxide nanoparticles having fluorescent substances. The system for detecting biomolecules can be reused after detection while simultaneously satisfying the reduction of a nonspecific binding and signal amplification using the rare-earth metal oxide nanoparticles having the Curie temperature near the body temperature.COPYRIGHT KIPO 2016