COMPOSITIONS AND METHODS FOR DETECTING RARE SEQUENCE VARIANTS IN NUCLEIC ACID SEQUENCING

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The present invention relates to compositions that include one or more control molecules known as artificial reference sequences and methods of using these control molecules for estimating rare nucleic acid sequence variants from low copy numbers in ultra-deep sequencing. The present invention is directed to compositions and methods for providing an in-process control for nucleic acid sequencing techniques, including, for example, next-generation sequencing (NGS) assays, to detect low-frequency sequence variants. These controls provide a number of technical advantages.