SELECTIVE AMPLIFICATION AND REAL-TIME PCR DETECTION OF RARE MUTATIONS

文件类型：PDF文档
文件大小：2639K
Provided herein are methods and kits for the improved detection of rare mutations within a high background. Exemplary embodiments relate to kits and methods that include amplification primers, a blocking oligonucleotide, and one or more allele-specific detector probes, useful in the specific detection of rare allelic variants or mutations.