METHOD OF PREPARING BONE TISSUE PREPARATION AND SET FOR ITS REALISATION

文件类型：PDF文档
文件大小：1022K
FIELD : medicine.SUBSTANCE : invention relates to medicine, veterinary and biology. Sample fixation, decalcification, washing with water, dehydration in alcohol solutions and pouring with paraffin are performed. Sample fixation is performed for 24 hours in alcohol-based molecular fixing agent FineFix, which contains FineFix and 96° alcohol in ratio 1 : 2.5. Decalcification is carried out for 2-5 days in 5-8% buffered solution of formic acid with daily replacement of decalcifying solution and control of decalcification completeness. Ratio sample : decalcifying solution constitutes 1 : 20. When decalcification is finished, sample washing with water is performed and sample is re-submerged into alcohol solution of molecular fixing agent FineFix for 6-12 hours before dehydration stage. Set for preparation of bone tissue preparation contains alcohol-based molecular fixing agent FineFix, concentrated solution of decalcifying agent, prepared with ratio 40 g of sodium citrate, 100 ml of 90% solution of formic acid, 300 ml of distilled water, and working solutions for control of decalcification completeness, containing saturated ammonium oxalate solution and 25% water solution of ammonia.EFFECT : invention makes it possible to obtain high-quality preparations suitable for further histological and immunohistological analysis without application of highly toxic components.2 cl, 2 dwg