ENZYME IMMUNOASSAY AND CHEMILUMINESCENT REAGENT KIT FOR ENZYME IMMUNOASSAY

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PROBLEM TO BE SOLVED : To provide an enzyme immunoassay and a chemiluminescent reagent kit for an enzyme immunoassay capable of measuring a very small amount of substances in a living body with high sensitivity. SOLUTION : There is provided a chemiluminescent enzyme immunoassay using a chemiluminescent substance (a) and an oxidant (b), wherein chemiluminescence is performed in the presence of a salt (c) of an alkali metal or an alkaline earth metal. The salt (c) of an alkali metal or an alkaline earth metal preferably is calcium chloride and/or lithium bromide. The enzyme preferably is a peroxidase and the chemiluminescent substance (a) preferably is a metal salt of luminol. COPYRIGHT : (C)2014, JPO&INPIT