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PURPOSE : A natural fertilizer using germinated barley and fresh water moss and a manufacturing method thereof are provided to control breeding of pests and eliminating all kinds of pathogens. CONSTITUTION : A manufacturing method of a natural fertilizer comprises the following steps : preparing fresh water moss powder; mixing the fresh water moss powder with water soaked barley in a ratio of 3 : 1 to 4 : 1; germinating the barley at 30-40deg.C in a system tray within a greenhouse for 15-16; cultivating sprouts at 30-40deg.C for 10 days until the sprout become 14-16cm; mixing the barley(90-95 weight%), crushed material of Acanthopanax sessiliflorum Seeman(5-10 weight%), half-crushed rice, and herbal medicine residue in one of the following ratios : 1 : 1 : 1, 1 : 1 : 1.2, 1 : 1.2 : 1 or 1.2 : 1 : 1; maturing after pouring water(60 parts) to the mixture at 30-45deg.C for 3 months; compress extracting the matured mixture from a compressor; and dividing liquefied fertilizer undiluted solution and the residue lumps which are solid natural fertilizer.