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PROBLEM TO BE SOLVED : To provide isolated high-purity X-chromosome bearing or Y-chromosome bearing populations of spermatozoa.
SOLUTION : There are provided : isolated non-naturally occurring populations of spermatozoa (15) having high purity; and technologies to differentiate sperm (28) based on characteristics such as mass, volume, orientation, or emitted light, including methods of analysis and apparatus such as beam shaping optics (30) and detectors (32). The isolated non-naturally occurring populations of spermatozoa having high purity have numerous applications including sex selection of offspring from mammals, various in vitro protocols for the fertilization of ova, various in vivo protocols such as artificial insemination, business methods involving the production of prize animals or meat animals, or preservation of rare or endangered animals, to recite but a few of the applications for high purity populations of spermatozoa.
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