DNA modular cloning vector plasmids and methods for their use

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(57) Abstract : Shortened abstract A group of modular cloning vector plasmids for the synthesis of transgenes or other complicated DNA constructs The invention is useful for assembling a variety of DNA fragments into a de/ ovo DNA construct by using cloning vectors optimized to reduce the amount of manipulation needed The module vector contains at ]east one multiple cloning site and multiple sets of rare restriction and/or unique homing endonuclease ("H") sites, arranged in a linear pattern This arrangement de- fines a modular architecture that allows the user to place domain modules or inserts into a PE3 transgene vector construct without disturbing the integrity of DNA elements already incorporated into the PE3 vector in previous cloning steps The PE3 transgenes roduced using the invention may be used in a variety of organisms including bacteria, yeast, mice, and other eukaryotes with little or no further modification before the expiration of the time limit for amending the For two-letter codes and other abbreviations, refer claims and to be republished in the event of receipt of ance Notes on Codes and Abbreviations" appearing amendments ning of each regular issue of the PCT Gazette. Date of publication of the international search report : 27 September 2007