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A modified T-ZnO whisker antibacterial agent and preparation method thereof are provided. The modified T-ZnO whisker antibacterial agent contains rare earth-modified tetrapod-like zinc oxide whisker. The preparation method comprises dispersing tetrapod-like ZnO whiskers and dispersant in dispersive medium, adding rare-earth metal compound under ultrasonic treatment, stirring to obtain suspended solution, filtering, washing and drying to obtain powdery modified T-ZnO whisker antibacterial materials. The T-ZnO whisker antibacterial materials have extended spectral response range and improved antibacterial effect.