A method of electrophoresis

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(57) Abstract : The present invention relates a method of electrophoretic separation of protein and/or peptide components of a sample in a convection stabilised medium. More specifically, the method comprises the steps to contact the sample with the separation medium; to apply a voltage across said medium : and to observe the results by analysis of one or more sections of the separation medium. In the present method, a disulphide comprising compound is added before or during the procedure to make an excess of reactive disulphide groups accessible to react with the cysteine groups of the proteins and/or peptides all through the separation procedure. The present invention also relates to electrophoretic separation media that comprises re-active disulphide groups, such as polyacrylamide gels, and the use of a solution that comprises reactive disulphide groups to pretreat an electrophoretic separation medium. For two-letter codes and other abbreviations, refer to ance Notes on Codes andAbbreviations" appearing ning of eaeh regular issue of the PCT Gazette.