DEVICES AND METHODS TO IMAGE OBJECTS BY TIME DELAY INTEGRATION

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Devices and methods for automated collection and image analysis are disclose d that enable identification or classification of microscopic objects aligned or deposited on surfaces. Such objects, e.g. detectably labeled rare target cells, are magnetically or non-magnetically immobilized and subjected to Tim e Delay Integration imaging (TDI). Incorporation of TDI technology into image cytometry analysis, like CellTracks.RTM., makes it possible to image moving objects with very high sensitivity and signal-to-noise ratios. Implementatio n of TDI camera technology with dual excitation and multispectral imaging of enriched rare cells provides a rapid system for detection, enumeration, differentiation and characterization of imaged rare cells on the basis of size, morphology and immunophenotype.