FLUORIMETER CELL AND ITS CALIBRATION

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A fluorimeter reference cell comprising a rare earth salt solution or other optically transparent solution with a well-defined and characteristic absorbance pattern which material emits an absorption spectrum when an excitation light is incident upon it. The absorption spectrum has a defined characteristic enabling wavelength calibration of the fluorimeter. The reference cell comprises a second material, a light scattering colloid such as colloidal silica. The dispersive properties result in the absorption spectrum being scattered or reflected at an angle to the angle of incidence of the excitation light incident on the reference cell.