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CELL DEVELOPMENT METHOD AND KIT FOR IT

2025-06-18 03:333220下载
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PROBLEM TO BE SOLVED : To provide a method by which rare cells can be efficiently recovered without disturbing adsorption to the surface of a substrate for cell development (preferably to the bottom face of a microchamber) while inhibiting adsorption to the surface of a laboratory instrument, using a substance treating cells. SOLUTION : The invention provides a cell development method comprising a cell immobilization treatment process, a protein removal process, and a cell development process, as well as fulfilling the following conditions (a)-(c) : (a) in the cell immobilization treatment process, a fixing agent consisting of a compound (glyoxal etc.) comprising two or more aldehyde groups in one molecule is used; (b) at least in a protein removal process and/or cell development process, a pipette tip and/or micro tube of a material selected from polypropylene etc. are used; (c) in a cell development process, a substrate for cell development made from polystyrene is used. SELECTED DRAWING : None COPYRIGHT : (C)2016, JPO&INPIT


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