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A live fish transportation vehicle includes a water tank (10) storing and transporting live fish and seawater; a seawater filtering chamber (10) including a filter (2) of a non-woven fabric or cotton form located on one side of the upper part of the water tank and a filter plate (3) supporting the filter; and a door (12) opened and closed on the upper part of the seawater filtering chamber (10), wherein a purifying unit (100) is detachably installed in the seawater filtering chamber (10) while the purifying unit (10) comprises a body (110), an injector (120), an aeration member (130), a bubble maintaining member (140), a guide member (150), and a seawater dividing member (160) to have an effect of not only providing fresh live fish, but also significantly reducing various kinds of the entire costs according to carrying of live fish.COPYRIGHT KIPO 2015