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Proceeded of regeneration of an enzymatic catalyst laid out in an engine including/understanding a mineral support containing alkaline and/or alkaline-earth oxide and at least of an enzyme characterized in that it contains at least a stage of unhooking of the enzymes used by solvation by sweeping of catalyst by means of to less one the surfactant ionic one, and at least a stage of accosting of the active enzymes by sweeping of the known as support purified by at least a solution of active enzymes, these two stages being made in-situ within the engine.