Methods and compositions for the specific inhibition of gene expression by double-stranded DNA

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(57) Abstract : The invention is directed to compositions and methods for selectively reducing the expression ofa gene product from a desired target gene in a cell, as well as for treating diseases caused by the expression of the gene. More particularly, the invention is directed to compositions that contain double stranded RNA ("dsRNA"), and methods for preparing them, that are capable of re- ducing the expression of target genes in eukaryotic cells. The dsRNA has a first oligonucleotide sequence that is between 25 and about 30 nucleotides in length and a second oligonacleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of at least 19 nucleotides is sufficiently com- lementary to a nucleotide sequence of the RNA produced tYom the target gene to trigger the destruction of the target RNA by the RNAi machinery. SE, SI, SK, TR), OAPI (BF, B J, CF, CG, CI, CM, GA, GN, For two-letter codes and other abbreviations, refer GQ, GW, ML, MR, NE, SN, TD, TG). ance Notes on Codes and Abbreviations" appearing ning of each regular issue of the PCT Gazette. Published : -- without international search report and to be republished upon receipt of that report