IDENTIFICATION AND USE OF PROGNOSTIC AND PREDICTIVE MARKERS IN CANCER TREATMENT

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The present invention provides a method of screening for markers useful in predicting the efficacy of a specified cancer that includes : (a) constructing a tissue microarray from a tissue bank comprising multiple tissue samples that are annotated with clinical follow up data; (b) labeling polynucleic acid probes specific for oncogenes or cancer associated genes known to be potential amplicons; (c) performing fluorescent in situ hybridization analysis on the tissue microarray; and (d) correlating the re-sult of the fluorescent in situ hybridization with the clinical follow up data. The present disclosure also provides methods of treat-ing breast cancer that include screening a breast cancer patient for amplification of one or more of the genes disclosed herein, and treating a patient having amplification of one or more of these genes with a therapeutically effective amount of a compound that interferes with HER2 signaling.