FED BATCH PROCESS FOR BIOCHEMICAL CONVERSION OF LIGNOCELLULOSIC BIOMASS TO ETHANOL

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A method for optimization of a fed-batch lignocellulosic biomass hydrolysis process wherein the hydrolysis time is minimized by controlling the feed addition volume and / or batch addition frequency of prehydrolysed lignocellulosic feed and optionally the enzyme feed. The increase over time in hydrolysate consistency and volume and / or concentration of sugars re-leased in the reactor, so that the enzymatic hydrolysis is controlled, significantly reduces the impact of cellulase feedback inhibi-tion, especially for enzyme contents lower than 0.5%. The overall time to reach conversion of the total prehydrolysate feed is re-duced significantly where the batch addition frequency is equal to one batch each time 70% to 90% of a theoretical cellulose to glucose conversion is reached, preferably when 80% conversion is reached. At an enzyme load of 0.3% in the reaction mixture, the optimum frequency each time 80% conversion was reached was found to be one batch every 105 minutes.