METHODS FOR QUANTITATING SMALL RNA MOLECULES

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In one aspect, the present in-vention provides methods, compositions and kits for amplifying a microRNA molecule to produce DNA molecules. The methods each include the steps of : (a) using primer exten-sion to make a DNA molecule that is comple-mentary to a target microRNA molecule; and (b) using a universal forward primer and a re-verse primer to amplify the DNA molecule to produce amplified DNA molecules. In some embodiments of the method, at least one of the forward primer and the reverse primer com-prise at least one locked nucleic acid molecule.